Ultra-diluted Toxicodendron pubescens attenuates pro-inflammatory cytokines and ROS- mediated neuropathic pain in rats

Perhaps the most important research on homeopathic medicine was just published at the website of the most respected scientific journal in the world (“Nature”).  

This vitally important group of studies have conclusively shown that homeopathic doses of Rhus toxicodendron (poison ivy) are significantly more effective than placebo in the treatment of neuropathy in rats.  Further, this research tested various doses of Rhux tox. and each potency, including 30X, was found to have profound measurable effects on neuropathy.  

Still further, this research shows that this homeopathic medicine had much of the same similar benefits as a conventional drug, Gabapentin but was found to be safer. There are presently over 300 clinical trials published in peer reivew medical journals, most of which show the efficacy of homeopathic treatment.  This new study confirms that homeopathic medicine, what I now call “the original nanomedicine,” have both biological effects and clinical efficacy.   

Ultra-diluted Toxicodendron  pubescens attenuates pro- inflammatory cytokines and ROS-  mediated neuropathic pain in rats 

Shital Magar1, Deepika Nayak2, Umesh B. Mahajan1, Kalpesh R. Patil1, Sachin D. Shinde1, Sameer N. Goyal3, Shivang Swaminarayan4, Chandragouda R. Patil1, Shreesh Ojha5 & Chanakya Nath Kundu2 

1Department of Pharmacology, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur- 425405, Dist. Dhule, Maharashtra, India. 2School of Biotechnology, Kalinga Institute of Industrial technology (a deemed to be University), Campus-11, Patia, Bhubaneswar, Odisha, Pin-751024, India. 3SVKM’s Institute of Pharmacy, Dhule-424001, Dist-Dhule, Maharashtra, India. 4Janmangal Homeopathy and Wellness Centre, Bopal, Ahmedabad, Gujarat, 380058, India. 5Department of Pharmacology & Therapeutics, College of Medicine & Health Sciences, UAE University, Al Ain, UAE. Correspondence and requests for materials should be addressed to C.R.P. (email: xplore. remedies@gmail.com) or S.O. (email: shreeshojha@uaeu.ac.ae) or C.N.K. (email: cnkundu@kiitbiotech.ac.in) 

Despite the availability of multiple therapeutic agents, the search for novel pain management of neuropathic pain is still a challenge. Oxidative stress and inflammatory signaling are prominently involved in clinical manifestation of neuropathic pain. Toxicodendron pubescens, popularly known as RhusTox (RT) is recommended in alternative medicines as an anti-inflammatory and analgesic remedy. Earlier, we reported anti-inflammatory, anti-arthritic and immunomodulatory activities of RhusTox. In continuation, we evaluated antinociceptive efficacy of RhusTox in the neuropathic pain and delineated its underlying mechanism. Initially, in-vitro assay using LPS-mediated ROS-induced U-87 glioblastoma cells was performed to study the effect of RhusTox on reactive oxygen species (ROS), anti-oxidant status and cytokine profile. Rhus Tox decreased oxidative stress and cytokine release with restoration of anti-oxidant systems. Chronic treatment with Rhus Tox ultra dilutions for 14 days ameliorated neuropathic pain revealed as inhibition of cold, warm and mechanical allodynia along with improved motor nerve conduction velocity (MNCV) in constricted nerve. Rhus Tox decreased the oxidative and nitrosative stress by reducing malondialdehyde (MDA) and nitric oxide (NO) content, respectively along with up regulated glutathione (GSH), superoxide dismutase (SOD) and catalase activity in sciatic nerve of rats. Notably, RhusTox treatment caused significant reductions in the levels of tumor necrosis factor (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β) as compared with CCI-control group. Protective effect of Rhus Tox against CCI-induced sciatic nerve injury in histopathology study was exhibited through maintenance of normal nerve architecture and inhibition of inflammatory changes. Overall, neuroprotective effect of Rhus Tox in CCI-induced neuropathic pain suggests the involvement of anti- oxidative and anti-inflammatory mechanisms. 

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